Pipetting users usually have many considerations when purchasing pipettes, such as accuracy, feel, brand, tip adaptability, etc. However, when choosing tips, users pay far less attention than when buying pipettes. Many of them spend a lot of money to buy imported pipettes but save money to buy disposable medical consumables. The quality of the tip will have a great influence on the pipetting accuracy.
Classification of pipette tips
As a consumable used in conjunction with pipettes, tips can generally be divided into standard tips, filter tips, and low adsorption tips according to different applications.
1. Standard tips are the most widely used tips, almost all pipetting operations can use ordinary tips, which are the most economical type of tips.
2. Filter tips are consumables designed to avoid cross-contamination, and are often used in experiments such as molecular biology, cytology, and virology.
3. For experiments with high sensitivity requirements, or precious samples or reagents that are prone to the residue, low adsorption tips can be selected to improve the recovery rate. The surface of the low adsorption tip has been treated with hydrophobicity, which can reduce the situation that the low surface tension liquid leaves more residue in the tip.
Wide mouth tip, ideal for absorbing viscous materials, genomic DNA, and cell culture fluid;
The performance index of the suction head: low adsorption, filter element, tightness, loading and ejecting force, no DNase and RNase, no pyrogen;
How to choose a good suction head?
"As long as the tip can be installed, it is the tip that can be used" - this is almost the common perception of all users about the suitability of the tip. This statement can be said to be partly true but not entirely true.
The tip that can be installed on the pipette can indeed form a pipetting system with the pipette to realize the pipetting function, but is it reliable? A question mark is required here. Answering this question requires data to speak.
1. You may wish to perform a performance test after matching the pipette with the tip. After rinsing the tip, repeat the sample addition operation several times, weigh the sample amount each time, and record the reading.
2. Calculate the accuracy and precision of the pipetting operation after converting the density of the test liquid into volume.
3. What we want to choose is a suction head with good precision. If the matching of the pipette and the tip is not accurate, it means that the seal between the pipette tips and the pipette cannot be guaranteed, making the results of each operation irreproducible.