What is PCR deep well plate
PCR (polymerase chain reaction) deep well plate is a laboratory plate used in molecular biology experiments for high-throughput PCR amplification of DNA samples. It is designed to hold multiple samples in a single well, and it is called a "deep well" plate because the wells are relatively deep, allowing larger sample volumes to be used compared to traditional PCR plates.PCR deep well plates
are usually made of high-quality polypropylene or polycarbonate with heat-sealed or adhesive lids to prevent contamination and evaporation during PCR. The plate can be used with a variety of PCR instruments and is compatible with standard PCR protocols.
PCR deep well plates are commonly used in research laboratories and applications requiring large-scale sample processing, such as DNA library preparation, high-throughput screening, and genotyping. It is also used for nucleic acid purification and storage, as the pores can be sealed to prevent contamination and evaporation.
Rough classification of PCR deep well plate
1. Classified by the number of holes, the more common ones can be divided into 96-well plates, 384-well plates, and 96-well I-shaped plates.
2. According to the hole type classification, the 96-well plate can be mainly divided into round hole type and square hole type. The 384-well plates are all square wells.
3. According to the shape of the bottom of the hole, there are two common types: U-shaped and V-shaped.PCR deep well plate
scope of use
1. Store samples:
It can replace conventional 1.5ml centrifuge tubes to store samples, and it is neatly placed during storage, saving space, with large storage capacity, and can withstand -80°C refrigerators. Therefore, it is also called a storage block.
2. Processing samples:
It can be combined with row guns, high-throughput automatic liquid manipulation instruments and software to achieve high-throughput operations on biological samples, such as protein precipitation and liquid-liquid extraction. Greatly improve the efficiency of sample processing. The PP material can withstand high temperature and high pressure sterilization at 121°C.
3. Sampling operation:
Commonly used in various autosamplers, it can be directly placed in the sample compartment of the autosampler to inject samples. Compared with traditional vials, it can not only double the number of samples placed in the sample compartment, but also realize the Samples are injected directly after processing on the 96-well plate, which saves the tedious work of drawing samples back and forth, arranging samples, capping, inserting intubation tubes, and washing bottles.