Do you know how to use embedding cassettes
Embedding cassettes are the process used to embed material powders or other bulk structures to provide performance support or chemical protection. Embedding is frequently used in immobilization materials for microorganisms, animal and plant cells. Enhance the quality of the treatment of microorganisms or cell tissues, thereby avoiding cutting and trimming.
When selecting materials, place the design blocks in disposable embedding cassettes with the design number written on them, cover the dehydration box, put them into the dehydration basket and enter the dehydration machine or manual dehydration. Store the investment counters in the lower shelf of the oven. When embedding, put the dehydrated tissue together with the dehydration basket in the same oven with the embedding bottom mold inside. Open the dehydration box cover, put the dehydration box cover aside, select the embedding bottom mold according to the size of the tissue block, heat it on the alcohol lamp with tweezers, clamp the tissue block and put it in the bottom mold, and write on the top cover tissue dehydration Arrange the disposable embedding cassettes
with block numbers, pour a little paraffin, it is advisable not to overflow, and leave it for a while.
Take out the ice box in the freezer of the refrigerator, put the embedded wax block in the ice box and freeze it for about 5 minutes to 10 minutes. The winter time is short and the summer time is longer. Take the wax block out of the bottom mold and collect the rest of the wax block. , remove the excess wax at both ends of the embedding cassettes, and slice them. The embedded bottom mold and dehydration box cover are sorted and put back into the oven for later use. Put the dehydration box cover in the dehydration basket, and rinse off the wax with hot water before the next use. This method is not only beneficial to the preservation of the wax block, but also saves paraffin.
Classification of common embedding cassettes
Embedding cassettes are used in histology laboratories to preserve and organize tissue specimens during embedding. There are several types of embedding cassettes, each designed to meet specific laboratory needs. Here are some of the most common types of embedding cassettes and their classifications:
Plastic embedding cassettes: These are the most commonly used embedding cassettes
in histology laboratories. They are made of a durable and chemically resistant plastic material, which makes them suitable for a wide variety of tissue samples.
Metal embedding cassettes: These embedding cassettes are made of metal and are mainly used for larger tissue samples. They are stronger than plastic cases and offer greater durability and stability.
Biopsy embedding cassettes: These embedding cassettes are designed for embedding small tissue specimens, such as biopsies. They are usually smaller in size and may have fine mesh or pores to allow for better fluid exchange during embedding.
HistoSET embedding cassettes: These embedding cassettes are plastic embedding cassettes with snap-on lids for increased sample security. They are available in a variety of colors to aid in specimen identification and tracking.
PTFE embedding cassettes: These embedding cassettes are made of a non-stick, chemical-resistant material called polytetrafluoroethylene (PTFE). They are typically used to process tissue specimens that are difficult to remove from traditional plastic boxes.
MegaSET embedding cassettes: These embedding cassettes are designed for large tissue samples. They are made of durable plastic material and have a large rectangular shape to accommodate samples up to 35 mm x 25 mm.
In conclusion, embedding cassettes can be classified according to their material, size and design features. Laboratories may choose to use different types of embedding cassettes depending on their specific needs and the type of tissue specimens they are processing.
What is the significance of embedding cassettes in histology?
In histology, embedding cassettes are used to fix tissue samples during paraffin embedding. The embedding process involves dehydrating, clearing, and infiltrating the tissue with molten paraffin in preparation for sectioning and staining.Embedding cassettes
are made of a porous material that allows the wax to penetrate the tissue sample evenly, ensuring that the tissue is fully embedded and supported by the wax block. Embedding cassettes also provide a safe and organized way to handle and store multiple tissue samples during embedding.
Once the tissue is embedded in wax, it can be sliced using a microtome and placed on glass slides for staining and microscopic examination. Waxes provide support for tissue sections and help maintain tissue structure and morphology during staining and mounting.
In conclusion, embedding cassettes are an essential tool in histology for properly embedding, cutting and staining tissue samples for microscopic examination.