Introduction to disposable cell filter funnel
A disposable cell filter funnel is a laboratory tool used to filter cell suspensions or solutions. It is usually made of plastic and designed for single-use application.
A disposable cell filter funnel usually consists of a filter unit and a collection unit. The filter unit has a filter membrane to retain cells and particles of a certain size. The collection unit is designed to collect the filtered solution.
The disposable nature of the filter funnel eliminates the need to clean and sterilize between uses, saving time and reducing the risk of contamination. Additionally, disposable filter funnels are often more cost-effective than reusable filter funnels in terms of purchase price and maintenance costs.
Disposable cell filter funnels are commonly used in a variety of applications, such as cell culture and microbiology, where maintaining a sterile environment and preventing contamination is important. They can also be used to filter liquids or gases in various laboratory environments.
A potential disadvantage of disposable cell filter funnels is their impact on the environment, as they generate plastic waste that must be disposed of properly. However, some manufacturers offer filter funnels made from biodegradable materials, which can reduce their environmental impact.
The steps used by the disposable cell filter funnel
Disposable cell strainer funnels are laboratory tools used to filter cell suspensions, tissue homogenates, and other biological samples. The steps to use the disposable cell filter funnel
are as follows:
Choose the right filter size: Choose the right filter size for the sample being filtered. Typically, 40 µm filters are used for filtering cell suspensions, while 100 µm filters are used for tissue homogenates.
Prepare the funnel: Remove the filter funnel from the packaging and attach it to a disposable vacuum bottle or vacuum pump.
Wet the filter: Wet the filter with an appropriate buffer or media to prevent clogging and improve sample flow through the filter.
Load the sample: Pour the sample into the filter funnel while gently applying vacuum pressure to force the sample through the filter.
Collect filtrate: The filtered sample or filtrate is collected in a thermos or collection tube attached to the funnel.
Disposal: After filtration, discard the filter funnel and collection bottles/tubes according to your laboratory's disposal protocol.
It is important to note that the filtration process should be done as quickly as possible to prevent damage to the cells or changes in the physiological properties of the sample. It is also important to follow the manufacturer's instructions for using disposable cell strainer funnels, including recommended pressure settings and filtration speeds.
What are the specifications of disposable cell filter funnel
Specifications for disposable cell strainer funnels can vary by manufacturer and intended use, but some common specifications include:
Filter Size: Disposable cell strainer funnels are available in different filter sizes from 20µm to 100µm. The proper filter size to use depends on the size of the cells or particles that need to be filtered.
Filter Material: The filter material used in disposable cell strainer funnels is typically made of polyethylene terephthalate (PET) or nylon, which are biocompatible and have low protein binding properties.
Volume: The volume of the disposable cell filter funnel
varies from 15mL to 150mL according to the size of the funnel.
Sterility: Disposable cell strainer funnels are usually sterilized using gamma radiation or ethylene oxide to ensure they are free of contaminants.
Compatibility: Disposable cell strainer funnels can be used with a variety of collection tubes or flasks and are generally designed to fit standard laboratory equipment such as pipettes and vacuum pumps.
Certification: Many disposable cell strainer funnels are certified to various regulatory standards such as ISO 9001 or ISO 13485, which ensures that the manufacturing process meets strict quality control standards.
It is important to check the manufacturer's specifications before selecting a disposable cell strainer funnel to ensure it is suitable for your specific application.