With the advancement of science and technology, embedding cassettes are gradually being widely used in many fields. Below, EKK Disposable Medical Consumables Company will take you to see how to use embedding cassettes.
An embedding cassette is a process used to embed a material powder or other bulk structure to provide performance support or chemical protection. Embedding is frequently used in immobilization materials for microorganisms, and animal, and plant cells. Enhance the quality of the treatment of microorganisms or cell tissues, thereby avoiding cutting and trimming.
When selecting materials, place the tissue block in the disposable embedding box with the tissue number written on it, cover the dehydration box cover, put it into the dehydration basket, and enter the dehydration machine or manual dehydration. Store the investment counters in the lower shelf of the oven. When embedding, put the dehydrated tissue together with the dehydration basket in the same oven with the embedding bottom mold inside. Open the dehydration box cover, put the dehydration box cover aside, select the embedding bottom mold according to the size of the tissue block, heat it on the alcohol lamp with tweezers, clamp the tissue block and put it in the bottom mold, and write on the top cover tissue dehydration Place a numbered disposable embedding box, pour a little paraffin, it is advisable not to overflow, and leave it for a while.
Take out the ice box in the freezer of the refrigerator, put the embedded wax block in the ice box, and freeze it for about 5 minutes to 10 minutes. The winter time is short and the summer time is longer. Take the wax block out of the bottom mold and collect the rest of the wax block. , remove the excess wax at both ends of the embedding cassette, and slice it. The embedded bottom mold and dehydration box cover are sorted and put back into the oven for later use. Put the dehydration box cover in the dehydration basket, and rinse off the wax with hot water before the next use. This method is not only beneficial to the preservation of the wax block but also saves paraffin.
The above is how to use the embedding cassette, I hope it will be helpful to everyone's study! Have you learned it?