What Problems Should Be Paid Attention To When Using Imported Cryovial Tubes?

　　Cryovial tubes, also known as strain preservation tubes, belong to disposable medical consumables. Commonly used methods for the preservation of strains in microbiology laboratories include the milk method, glycerin method, slope method, etc. The complexity varies and the effects vary widely. At present, in most domestic laboratories, the experimenters make the strain preservation tubes by themselves, which not only increases the work intensity but also the effect of strain preservation cannot always be satisfactory due to the limitations of various conditions. Let me give you a detailed introduction to the use and precautions of imported cryovial tubes.

　　The use of imported cryovial tubes is a science, not a simple trilogy such as opening the liquid nitrogen tank, putting in the cryovial tubes, and closing the liquid nitrogen tank. Using cryovial tubes scientifically and correctly can avoid the loss of samples and protect the safety of experimenters. Therefore, we should pay attention to the following issues when using it:

　　1. It needs to be operated in a biological safety cabinet to ensure the sterility of the operation and ensure that the strains are not contaminated.

　　2. The cryovial tubes should not be used if the following conditions occur before inoculation: the bottle leaks. The cryopreservation solution in the cryovial tubes is cloudy (indicating contamination). beyond the effective storage period.

　　3. When discarding used or partially used cryovial tubes, attention should be paid to the prevention of biological hazards.

　　4. When using cryovial tubes to store samples, it is strictly required to store the cryovial tubes in the gas phase layer of liquid nitrogen or the refrigerator. If the cryovial tubes are stored in liquid nitrogen, there is a certain chance that the liquid nitrogen will seep into the cryovial tubes. During resuscitation, the gasification of the liquid nitrogen will cause the pressure inside and outside the tube to be unbalanced, which will easily cause the bursting of the cryovial tubes and be biohazardous.

　　5. When resuscitating imported cryovial tubes, use safety protection equipment throughout the process. It is recommended to wear lab coats and cotton gloves and operate on a safe laboratory bench. Wear goggles or a face shield when conditions permit. Please be extra careful as the indoor temperature will be higher in summer than in winter.