1. Use appropriate tips: To ensure better accuracy and precision, it is recommended that the pipetting volume is within the range of 35%-100% of the tip's volume.
2. Tip installation: For most brands of pipettes, especially multi-channel pipettes, it is not easy to install the tip: to achieve a good seal, you need to insert the pipette handle into the tip, turn it left and right or Rock back and forth to tighten firmly. Some people will use the pipette to hit the tip repeatedly to tighten it, but this operation will cause the deformation of the tip and affect the accuracy, and in severe cases, it will damage the pipette, so such an operation should be avoided.
3. Tip immersion angle and depth: The tip immersion angle is controlled within 20 degrees of inclination, and it is better to keep it vertical;
For samples at room temperature, rinsing with pipette tips can help improve accuracy; but for samples with high or low temperature, rinsing with pipette tips will reduce the accuracy of operation, please pay special attention to users.
5. Aspirating speed: The pipetting operation should maintain a smooth and appropriate aspirating speed; too fast aspirating speed will easily cause the sample to enter the sleeve handle, causing damage to the piston and sealing ring and cross-contamination of the sample.
1. Maintain the correct posture when pipetting; do not hold the pipette tightly all the time, use a pipette with a finger hook to help relieve hand fatigue; change hands frequently if possible.
2. Regularly check the sealing condition of the pipette. Once the sealing is found to be aging or leaking, the sealing ring must be replaced in time.
3. Calibrate the pipette 1-2 times a year (depending on the frequency of use).